Gated STED microscopy with time-gated single-photon avalanche diode

Two-Photon Excitation STED Microscopy with Time-Gated Detection

We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE-STED microscope with a stimulated emission beam running in a continuous-wave. Th...

Actively quenched single-photon avalanche diode for high repetition rate time-gated photon counting

This paper reports an experimental characterization of the EG&G SPCM-AQ single-photon avalanche diode module with an active quenching bias circuit that gives a dead time of ;35 ns for use in high count rate, fast timing applications. A quantum efficiency of .70% and an optimal timing jitter with a full width at one-thousandth maximum of 1.5 ns is obtained if the light is tightly focused to a sp...

Memory effect in silicon time-gated single-photon avalanche diodes

Non-line-of-sight imaging using a time-gated single photon avalanche diode.

By using time-of-flight information encoded in multiply scattered light, it is possible to reconstruct images of objects hidden from the camera's direct line of sight. Here, we present a non-line-of-sight imaging system that uses a single-pixel, single-photon avalanche diode (SPAD) to collect time-of-flight information. Compared to earlier systems, this modification provides significant improve...

FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting

MOTIVATION Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility and how mobility relates to molecular interaction dynamics and bioactivity in living cells. The FCS technique has been significantly advanced by its combination with super-resolution STED microscopy (STED-FCS). Specifically, the use of gated detection has shown great potential for enhancing...